Purification and complete amino acid sequence of canine pancreatic secretory trypsin inhibitor

FEBS Letters

Nagata, Kiyoshi²  Yoshida, Nobuo²  Saitoh, Yohichi¹  Kikuchi, Norihisa²  Yamamoto, Masahiro¹  Tanaka, Tatsuhiko¹ 

[1] First Department of Surgery,Kobe University School of Medicine, 7-5-1 Kusunokicho, Chuo-ku, Kobe 650, Japan;Shionogi Research Laboratories, Shionogi & Co., Ltd. 5-12-4 Sagisu, Fukushima-ku, Osaka 553, Japan

关键词: Trypsin inhibitor;    Amino acid sequence;    tHPLC;    Canine pancreatic juice;    Bovine trypsin Sequence homology;    PSTI;    pancreatic secretory trypsin inhibitor;    RIA;    radioimmunoassay;    SPase;    Staphylococcus aureus V8 protease;   

DOI  :  10.1016/0014-5793(85)80022-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pancreatic secretory trypsin inhibitor (PSTI) was purified from canine pancreatic juice by HPLC. Canine PSTI inhibited bovine trypsin activity stoichiometrically and strongly with a dissociation constant of below 10⁻⁹ M. The amino acid sequence of canine PSTI was determined by conventional methods. It had one more amino acid residue at the amino-terminus than other mammalian PSTIs, i.e. human, porcine, bovine and ovine.

【 授权许可】

Unknown