Epidermal growth factor‐induced increases in inositol trisphosphates, inositol tetrakisphosphates, and cytosolic Ca2+ in a human hepatocellular carcinoma‐derived cell line

FEBS Letters

Prentki, Marc¹  Gilligan, Ann²  Knowles, Barbara B.²  Glennon, Major C.¹ 

[1] Department of Biochemistry and Biophysics and Diabetes Research Center, University of Pennsylvania, Philadelphia, PA 19104, USA;Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104 USA

关键词: Epidermal growth factor;    Inositol phosphate metabolism;    Cytosolic Ca2+;    Phosphatidylinositol 4;    5-bisphosphate hydrolysis;    (Human hepatoma cell;    PLC/PRF/5);   

DOI  :  10.1016/0014-5793(88)81352-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A human hepatocellular carcinoma-derived cell line, PLC/PRF/5, was examined for its ability to respond to epidermal growth factor (EGF) exposure with increased phosphatidylinositol 4,5-bisphosphate hydrolysis. Upon addition of EGF (25 ng/ml) a rapid (10–15 s) but transient increase in Ins(1,4,5)P₃ levels and large, prolonged (2 min) increases in Ins(1,3,4,5)P₄ andIns(1,3,4)P₃ levels were detected. Increases in cytosolic Ca²⁺ were observed after a 10 to 2O s lag, reaching peak value at 1 min, and remaining elevated for 10 min. The initial burst of cytosolic Ca²⁺ occurred in the absence of extracellular Ca²⁺ and probably reflects mobilization of intracellular Ca²⁺ stores. In cells pretreated with EGTA, the sustained component of the Ca²⁺ response was not observed. Comparison of the inositol phosphate and Ca²⁺ responses of PLC/PRF/5 cells to responses reported in other cell types indicates that this cell line is a good model for EGF action in liver.

【 授权许可】

Unknown