Cloning and expression of human brain type I inositol 1,4,5‐trisphosphate 5‐phosphatase High levels of mRNA in cerebellar Purkinje cells

FEBS Letters

Mailleux, Pierre²  Erneux, Christophe¹  Verjans, Benoît¹  De Smedt, Florence¹ 

[1] Interdisciplinary Research Institute (IRIBHN) Université Libre de Bruxelles, Campus Erasme, Bldg. C 808 route de Lennik, 1070 Brussels, Belgium;Brain Research Unit, Université Libre de Bruxelles, Campus Erasme, Bldg. C 808 route de Lennik, 1070 Brussels, Belgium

关键词: Inositol phosphate metabolism;    Signal transduction;    Ca2+;    Human brain;   

DOI  :  10.1016/0014-5793(94)00509-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In brain and many other tissues, Type I inositol 1,4,5-trisphosphate (InsP₃) 5-phosphatase is the major isozyme hydrolysing the calcium-mobilizing second messenger InsP₃. We recently reported the cloning and expression of dog thyroid InsP₃ 5-phosphatase. During the course of this cloning, screening of a human brain cDNA library allowed us to isolate a cDNA clone D1 with 91% sequence identity with the thyroid sequence. When clone D1 was expressed in Escherichia coli, the fusion protein had InsP₃ 5-phosphatase activity. M _r estimates of the recombinant enzyme made by unmunodetection, activity assay after SDS/PAGE or silver staining were consistent with the calculated molecular mass. In situ hybridization on human cerebellum sections localised the mRNA for this enzyme to the Purkinje cells.

【 授权许可】

Unknown