Phosphatidylinositol 4,5‐bisphosphate stimulates protein kinaseC‐mediated phosphorylation of soluble brain proteins

FEBS Letters

Chauhan, Ved P.S.¹ 

[1] New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, USA

关键词: Neomycin;    Protein kinase C;    Phosphatidylinositol 4;    5-bisphosphate;    DG;    diacylglycerol;    PIP2;    Phosphatidylinositol 4;    5-bisphosphate;    PKC;    protein kinase C;    PS;    phosphatidylserine;   

DOI  :  10.1016/0014-5793(90)80457-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphatidylinositol 4,5-bisphosphate (PIP₂) at 0.1 mol% activated the protein kinase C (PKC)-mediated phosphorylation of 87-, 55- and 47-kDa brain proteins. Neomycin, an aminoglycoside antibiotic that binds PIP₂ with a high affinity, inhibited PIP₂·PKC activity in a concentration-dependent manner. Low concentrations of neomycin (< 2 mM) did not affect DG·PKC activity; however, 4 mM neomycin inhibited 50% of this activity. This inhibition of DG-stimulated activity at high neomycin concentration may be the result of its binding to Ca²⁺ and ATP in the assay system. These results suggest that PIP₂ is a physiological activator of PKC, and show that neomycin can be an inhibitor of PIP₂·PKC activity at concentrations where DG·PKC activity is not affected.

【 授权许可】

Unknown