A photoactivatable synthetic transit peptide labels 30 kDa and 52 kDa polypeptides of the chloroplast inner envelope membrane

FEBS Letters

Kaderbhai, Mustak A.¹  Kaderbhai, Naheed¹  Austen, Brian M.²  Pickering, Tracy¹ 

[1] University College of Wales, Penglais, Aberystwyth SY23 3DD, Wales England;Department of Surgery, St George's Hospital Medical School, Cranmer Terrace, London SW17 ORE, England

关键词: Transit peptide;    Chloroplast;    Import receptor;    ASTP;    Met1-(4-azidosalicyl);    Lys20-(4-azidosalicyl) transit peptide;    IEF;    isoelectric focussing;    PAGE;    polyacrylamide gel electrophoresis;    Rubisco;    ribulose-1;    5-bisphosphate carboxylase;   

DOI  :  10.1016/0014-5793(88)80760-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 24-residue transit peptide based on the sequence of a precursor of the small subunit of wheat ribulose-1,5-bisphosphate carboxylase (Rubisco) was synthesized. The transit peptide was converted into a radioactive azido derivatised analogue. Photoactivation of the radiolabelled transit peptide analogue with isolated inner and outer membranes of the chloroplast envelope intensely labelled two proteins of 30 kDa and 52 kDa. In the outer membrane only the 52 kDa polypeptide was labelled. These findings are consistent with a recent report on the identification of the 30 kDa receptor protein for protein import in the chloroplast envelope contact zones [(1988) Nature 331, 232–237].

【 授权许可】

Unknown