Initial and sustained calcium mobilizations in the parietal cell during stimulations with gastrin, inositol trisphosphate, phorbol ester and erogenous diacylglycerol

FEBS Letters

Takeda, Hiroshi³  Tsunoda, Yasuhiro¹  Asaka, Masahiro³  Nakagaki, Ikuko²  Sasaki, Sadao² 

[1] Department of Laboratory Medicine, Hokkaido University, School of Medicine, Sapporo, Hokkaido 060, Japan;Department of Physiology, Osaka Medical College, Takatsuki, Osaka 569, Japan;3rd Department of Internal Medicine, Hokkaido University, School of Medicine, Sapporo, Hokkaido 060, Japan

关键词: Ca2+ mobilization;    Gastrin;    Inositol trisphosphate;    Diacyglycerol;    (Guinea pig parietal cell);    fura-2;    Mops;    3-(N-morpholino)propanesulfonic acid;   

DOI  :  10.1016/0014-5793(88)80391-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Electron probe X-ray microanalysis revealed that cytoplasmic Ca²⁺ concentration increased in the restricted apical cytoplasm during stimulation of isolated guinea pig parietal cells with gastrin. Furthermore, this study, using ⁴⁵Ca²⁺, aequorin and fura-2, revealed the mechanism involved in intracellular Ca²⁺ shifts caused by gastrin and the involvements of inositol 1,4,5-trisphosphate (IP₃) and diacylglycerol in producing those shifts. The gastrin-mediated and IP₃-sensitive Ca²⁺ pool was located in the smooth-surfaced membrane-enriched areas and released Ca²⁺ in the initial phase. Gastrin-mediated Ca²⁺ mobilization was also evoked by diacylglycerol, comprising an intracellular Ca²⁺ mobilization followed by a late, sustained and more localised Ca²⁺ entry from the extracellular space.

【 授权许可】

Unknown