Photoaffinity labelling shows that Escherichia coli isocitrate dehydrogenase kinase/phosphatase contains a single ATP‐binding site

FEBS Letters

Nimmo, Hugh G.¹  Varela, Isabel¹ 

[1] Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland

关键词: Photoaffinity labeling;    8-AzidoATP;    Bifunctional enzyme;    Isocitrate dehydrogenase kinase/phosphatase;    ICDH;    isocitrate dehydrogenase;    FPLC;    fast protein liquid chromatography;    Mops;    4-morpholinepropane-sulphonic acid;   

DOI  :  10.1016/0014-5793(88)80850-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ultraviolet irradiation of E.coli isocitrate dehydrogenase kinase/phosphatase in the presence of 8-azidoATP resulted in parallel losses of its kinase and phosphatase activities, and in covalent attachment of the reagent to the protein at a single site. ATP and ADP protected the two activities to similar extents. The data suggest that the activation of the phosphatase by adenine nucleotides results from binding of the nucleotides to the active site of the kinase.

【 授权许可】

Unknown