3β,17β‐Hydroxysteroid dehydrogenase of Pseudomonas testosteroni

FEBS Letters

Legoy, Marie-Dominique¹  Minard, Philippe¹  Thomas, Daniel¹ 

[1] Laboratoire de Technologie Enzymatique, Université de Technologie de Compiègne, BP 233, 60206 Compiègne, France

关键词: 3β1 7β-Hydroxysteroid dehydrogenase;    δ5-3-Ketosteroid isomerase;    Bifunctional enzyme;    Dehydroepiandrosterone;    Pseudomonas testosteroni;    NAD regeneration;    isomerase;    Δ5-3-ketosteroid isomerase;    DHEA;    dehydroepiandrosterone (3β-hydroxy-17-oxoandrost-5-ene);    YADH;    yeast alcohol dehydrogenase;   

DOI  :  10.1016/0014-5793(85)80880-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

3β,17β-Hydroxysteroid dehydrogenase (3β17/βHSDH) is an NAD-dependent dehydrogenase which has a double specificity for the 3- and 17-positions on the steroid skeleton. When dehydroepiandrosterone (DHEA) is used as steroid substrate, and the assay coupled with ketosteroid-isomerase, the two reactions occur alternately and each reaction on the 3-position produces a chromophoric molecule. These two reactions can follow one another without dissociation of the coenzyme from the enzyme binding site. This is confirmed by competition experiments with another dehydrogenase.

【 授权许可】

Unknown