Number of tRNA binding sites on 80 S ribosomes and their subunits

FEBS Letters

Semenkov, Yu.P.²  El'skaya, A.V.¹  Kirillov, S.V.²  Rodnina, M.V.¹ 

[1] Institute of Molecular Biology and Genetics, USSR Academy of Sciences, ul. Zabolotnogo, 24, Kiev 252627 USSR;Konstantinov Nuclear Physics Institute, USSR Academy of Sciences, Gatchina, Leningrad district 188350, USSR

关键词: 80 S ribosome;    40 S subunit;    60 S subunit;    tRNA binding site;   

DOI  :  10.1016/0014-5793(88)80705-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ability of rabbit liver ribosomes and their subunits to form complexes with different forms of tRNA^Phe (aminoacyl-, peptidyl- and deacylated) was studied using the nitrocellulose membrane filtration technique. The 80 S ribosomes were shown to have two binding sites for aminoacyl- or peptidyl-tRNA and three binding sites for deacylated tRNA. The number of tRNA binding sites on 80 S ribosomes or 40 S subunits is constant at different Mg²⁺ concentrations (5–20 mM). Double reciprocal or Scatchard plot analysis indicates that the binding of Ac-Phe-tRNA^Phe to the ribosomal sites is a cooperative process. The third site on the 80 S ribosome is formed by its 60 S subunit, which was shown to have one codon-independent binding site specific for deacylated tRNA.

【 授权许可】

Unknown