Peptidyltransferase center of ribosomes

FEBS Letters

Krayevsky, Alexander¹  Victorova, Ljubov¹  Kukhanova, Marina¹ 

[1] Institute of Molecular Biology, USSR Academy of Sciences, Vavilov str., 32, Moscow-334, 117984, USSR

关键词: Alkaloid lycorine;    80 S ribosome;    Mechanism of action;    Association constant;    Peptidyltranferase center;    PTC;    the peptidyltransferase center;    CACCA-Phe and CACCA-Leu←Ac;    3′-terminal fragments of Phe-tRNA and AcLeu-tRNA;    respectively;    pA-Met←f;    3′ (2′)-O-(N-formylmethionyl)-adenosine 5′-phosphate;    the minimal model donor;    pC;    cytidine 5′-phosphate;   

DOI  :  10.1016/0014-5793(83)80951-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The molecular mechanism of action of the alkaloid lycorine has been revised. According to our results, lycorine inhibits the binding of CACCA-Leu←Ac to the donor site of the peptidyltranferase center of wheat-germ ribosomes, whereas the transpeptidation reaction in the system with the minimal model donor is not inhibited. The equilibrium constant of CACCA-Leu←Ac to the donor site of 80 S ribosomes is measured.

【 授权许可】

Unknown