Role of acidic phosphoproteins in the partial reconstitution of the active 60 S ribosomal subunit

FEBS Letters

Reboud, Anne-Marie¹  Reboud, Jean-Paul¹  Lavergne, Jean-Pierre¹  Conquet, François¹ 

[1] Laboratoire de Biochimie Médicale, Université Lyon I, UFR Lyon-Nord, 43, Boulevard du 11 Novembre 1918, Villeurbanne, France

关键词: Ribosome;    60 S subunit;    Phosphoprotein;    Reconstitution;   

DOI  :  10.1016/0014-5793(87)80761-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have recently shown that rat liver 60 S ribosomal subunits active in protein synthesis can be reconstituted from inactive core particles lacking 30% of the total proteins, mainly L10a, L12, L22, L24, A33 and the acidic phosphoproteins P1–P2, obtained by treatment of 60 S subunits with dimethylmaleic anhydride [(1987) Eur. J. Biochem. 163, 15–20]. In this study, an ethanol extract of the 60 S subunit which contains only P1–P2 was also shown to be effective in reconstitution with the DMMA-core-particies: it strongly stimulated the EF-2-dependent GTP hydrolysis and, to a lesser extent, polyphenylalanine synthesis; like the DMMA wash it shifted the thermal denaturation curve of the DMMA-core particles towards that of control subunits. Prior dephosphorylation of the ethanol extract by alkaline phosphatase inhibited the reconstruction process.

【 授权许可】

Unknown