Base‐pair formation between 18 S ribosomal RNA and globin mRNA during initiation of protein synthesis in vitro

FEBS Letters

Erni, Bernhard²  Staehelin, Theophil¹ 

[1] Hoffmann—La Roche, Research Division, Basel, Switzerland;Basel Institute for Immunology, Basel Switzerland

关键词: Base pairing;    Initiation factor;    mRNA—18 S rRNA interaction;    Protein synthesis;    eukaryotic;    Reconstitution;    in vitro;    Ribosome;    eIF;    eukaryotic initiation factor;    LDS;    lithium dodecylsulfate;    Hepes;    4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid;   

DOI  :  10.1016/0014-5793(82)81246-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A stable complex between 18 S rRNA and globin mRNA has been isolated from 40 S initiation complexes in the reconstituted reticulocyte cell free system. This complex is only formed under the conditions which also lead to an initiation complex active in protein synthesis. The mRNA—18 S rRNA interactions has properties compatible with base-pairing. This observation is discussed in the context with other, in part controversial, observations relating to base pairing as a step in initiation of eukaryotic protein synthesis.

【 授权许可】

Unknown