FEBS Letters | |
Fluorescence digital image analysis of the inositol trisphosphate‐mediated calcium transient in single permeabilized parietal cells | |
Yodozawa, Susumu1  Tsunoda, Yasuhiro1  Tashiro, Yutaka1  | |
[1] Department of Physiology, Kansai Medical University, Moriguchi, Osaka 570, Japan | |
关键词: Fluorescence digital imaging; Ca2+ transient; Inositol trisphosphate; Permeabilized cell; (Parietal cell; Guinea pig); fura-2; 1; (2-(5′-carboxyoxazol-2′-yl)-6-amino-benzofuran-5-oxy)-2-(2′-amino-5′-methylpheoxy)ethane-N; N; N′; N′-tetraacetic acid; Mops; 3-(N-morpholino)propanesulfonic acid; | |
DOI : 10.1016/0014-5793(88)80696-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The myo-inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ mobilization in single saponin-permeabilized and fura-2-loaded parietal cells was analysed by a fluorescence digital image processor. When the cells were incubated with ATP, free cytoplasmic Ca2+ concentration ([Ca2+]i) increased in some restricted cytoplasmic regions showing discontinuous plateau and in the peripheral cytoplasm showing continuous [Ca2+]i gradient towards the plasma membranes. When treated with IP3, the high plateau enlarged to the entire cytoplasm and (a) new higher plateau(s) appeared and enlarged again in a transient manner. The IP3-induced Ca2+ transient was also observed by fluorescence microphotometry of the single cells or by fluorescence spectrophotometry and 45Ca2+ uptake experiment of the cell population.
【 授权许可】
Unknown
【 预 览 】
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