Fluorescence digital image analysis of the inositol trisphosphate‐mediated calcium transient in single permeabilized parietal cells

FEBS Letters

Yodozawa, Susumu¹  Tsunoda, Yasuhiro¹  Tashiro, Yutaka¹ 

[1] Department of Physiology, Kansai Medical University, Moriguchi, Osaka 570, Japan

关键词: Fluorescence digital imaging;    Ca2+ transient;    Inositol trisphosphate;    Permeabilized cell;    (Parietal cell;    Guinea pig);    fura-2;    1;    (2-(5′-carboxyoxazol-2′-yl)-6-amino-benzofuran-5-oxy)-2-(2′-amino-5′-methylpheoxy)ethane-N;    N;    N′;    N′-tetraacetic acid;    Mops;    3-(N-morpholino)propanesulfonic acid;   

DOI  :  10.1016/0014-5793(88)80696-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The myo-inositol 1,4,5-trisphosphate (IP₃)-induced Ca²⁺ mobilization in single saponin-permeabilized and fura-2-loaded parietal cells was analysed by a fluorescence digital image processor. When the cells were incubated with ATP, free cytoplasmic Ca²⁺ concentration ([Ca²⁺]_i) increased in some restricted cytoplasmic regions showing discontinuous plateau and in the peripheral cytoplasm showing continuous [Ca²⁺]_i gradient towards the plasma membranes. When treated with IP₃, the high plateau enlarged to the entire cytoplasm and (a) new higher plateau(s) appeared and enlarged again in a transient manner. The IP₃-induced Ca²⁺ transient was also observed by fluorescence microphotometry of the single cells or by fluorescence spectrophotometry and ⁴⁵Ca²⁺ uptake experiment of the cell population.

【 授权许可】

Unknown