Biosynthesis of sialosyllactotetraosylceramide in human colorectal carcinoma cells

FEBS Letters

Liepkans, Vis¹  Jolif, Alain¹ 

[1] IRSC-CNRS, ER 277, Villejuif, France

关键词: Glycolipid;    Ganglioside;    Transferase;    Sialidase;    (Carcinoma cell);    HPTLC;    high performance thin-layer chromatography;    Mab;    monoclonal antibody;    IV3-NeuNAcLcOse4Cer;    NeuNAc(α2→3)Gal(β1→3)GlcNAc(β1→3)Gal(β1→4)GlcCer (Nomenclature of glycolipids according to the IUPAC-IUB commission on biochemical nomenclature [(1977) Eur. J. Biochem. 79;    11–21]);   

DOI  :  10.1016/0014-5793(88)80659-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A monosialoganglioside, IV³-NeuNAcLcOse₄Cer, has recently been detected in colorectal carcinoma cells, small cell lung carcinoma cells, embryonal carcinoma cells and in human brain extracts. We report here the presence of a CMP-sialic: LcOse₄Cer sialyl transferase activity in subcellular membrane fractions of the human colorectal carcinoma, SW1116, which recognizes the non-reducing terminal galactosyl moiety of lactotetraosylceramide. A convenient method for structural analysis of picomolar quantities of the radioactive enzymatic product(s) using bacterial endoglycoceramidase, sialidase and a viral sialidase is presented.

【 授权许可】

Unknown