Evidence for sialidase hydrolyzing gangliosides GM2 and GM1 in rat liver plasma membrane

FEBS Letters

Miyagi, Taeko¹  Tsuiki, Shigeru¹ 

[1] Biochemistry Laboratory, Research Institute for Tuberculosis and Cancer, Tohoku University, 4-1 Seiryomachi, Sendai 980, Japan

关键词: Sialidase;    Ganglioside;    (Rat liver);    Plasma membrane;    NeuAc;    N-acetylneuraminic acid;    UDP-Gal;    UDP-galactose;    UDP-GalNAc;    UDP-N-acetyl-galactosamine;   

DOI  :  10.1016/0014-5793(86)80985-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat liver plasma membrane removed sialic acid from mixed bovine brain gangliosides more efficiently than from sialyllactose and orosomucoid with an optimal pH of 4.5. When individual gangliosides, each labeled with [¹⁴C]sialic acid or [³H]sphingosine, were tested, not only GD_la and GM₃ but also GM₂ and GM₁, both of which had been considered to resist mammalian sialidases, were desialylated. The products of GM₂ and GM₁ hydrolysis were identified as asialo-GM₂ and asialo-GM₁, respectively, by thin-layer chromatography.

【 授权许可】

Unknown