Overexpression of cytosolic sialidase Neu2 induces myoblast differentiation in C2C12 cells

FEBS Letters

Colombo, F.²  Preti, A.²  Zizioli, D.²  Giuliani, R.²  Marchesini, S.²  Fanzani, A.²  Presta, M.¹ 

[1] Unit of General Pathology and Immunology, Department of Biomedical Sciences and Biotechnology, University of Brescia, Brescia, Italy;Unit of Biochemistry, Department of Biomedical Sciences and Biotechnology, University of Brescia, Brescia, Italy

关键词: Sialidase;    Myoblast;    Differentiation;    Myogenin;    Ganglioside;    PCR;    polymerase chain reaction;    4-MUB-NANA;    4-methylumbelliferyl N-acetylneuraminic acid;    bHLH factors;    basic helix loop helix factors;   

DOI  :  10.1016/S0014-5793(03)00709-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cytosolic sialidase Neu2 has been implicated in myoblast differentiation. Here we observed a significant upregulation of Neu2 expression during differentiation of murine C2C12 myoblasts. This was evidenced both as an increase in Neu2 mRNA steady-state levels and in the cytosolic sialidase enzymatic activity. To understand the biological significance of Neu2 upregulation in myoblast differentiation, C2C12 cells were stably transfected with the rat cytosolic sialidase Neu2 cDNA. Neu2 overexpressing clones were characterized by a marked decrement of cell proliferation and by the capacity to undergo spontaneous myoblast differentiation also when maintained under standard growth conditions. This was evidenced by the formation of myogenin-positive myotubes and by a significant decrease in the nuclear levels of cyclin D1 protein. No differentiation was on the contrary observed in parental and mock-transfected cells under the same experimental conditions. The results indicate that Neu2 upregulation per se is sufficient to trigger myoblast differentiation in C2C12 cells.

【 授权许可】

Unknown