The triiodothyronine nuclear receptor c‐ErbAα1 inhibits avian MyoD transcriptional activity in myoblasts

FEBS Letters

Wrutniak-Cabello, Chantal¹  Daury, Laetitia¹  Busson, Muriel¹  Cabello, Gérard¹  Cassar-Malek, Isabelle¹  Casas, François¹ 

[1] Unité d'Endocrinologie Cellulaire, UMR Différenciation Cellulaire et Croissance (INRA, Université Montpellier II, ENSAM), Institut National de la Recherche Agronomique (INRA), 2 place Viala, 34060 Montpellier Cedex 1, France

关键词: T3 nuclear receptor;    MyoD;    Myogenin;    Myoblast;    Differentiation;   

DOI  :  10.1016/S0014-5793(01)03063-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Thyroid hormone stimulates myoblast differentiation, through an inhibition of AP-1 activity occurring at the onset of differentiation. In this study we found that the T3 nuclear receptor c-ErbAα1 (T3Rα1) is involved in a mechanism preserving the duration of myoblast proliferation. Independently of the hormone presence, T3Rα1 represses avian MyoD transcriptional activity. Using several mutants of T3Rα1, we found that the hinge region plays a crucial role in the inhibition of MyoD activity. In particular, mutations of two small basic sequences included in α helices abrogate the T3Rα1/MyoD functional interaction. Similarly, the T3 receptor also represses myogenin transcriptional activity. Therefore, despite stimulating avian myoblast differentiation by a T3-dependent pathway not involving myogenic factors, T3Rα1 contributes to maintain an optimal myoblast proliferation period by inhibiting MyoD and myogenin activity.

【 授权许可】

Unknown