Complete cDNA sequence of a Dictyostelium ubiquitin with a carboxy‐terminal tail and identification of the protein using an anti‐peptide antibody

FEBS Letters

Westphal, M.¹  Jaeger, E.¹  Gerisch, G.¹  Noegel, A.¹  Müller-Taubenberger, A.¹ 

[1] Max-Planck-Institut für Biochemie, 8033 Martinsried bei München, FRG

关键词: Ubiquitin;    Anti-peptide antibody;    (Dictyostelium);   

DOI  :  10.1016/0014-5793(88)81139-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete sequence of a Dictyostelium discoideum cDNA is presented that codes for monoubiquitin extended at its C-terminus by a 52 amino acid tail. The sequence of both the ubiquitin portion and the tail is highly homologous to the one of Saccharomyces cerevisiae and to a partial mouse sequence. The highly basic tail sequence contains a putative metal and nucleic acid-binding motif. The gene encoding the 0.6 kb mRNA of the C-terminally extended ubiquitin is represented only once in the haploid genome. The 0.6 kb mRNA as well as its translation product, a 15 kDa protein, is expressed in exponentially growing cells and remains present for at least 5 h of development. Using antibodies against a synthetic peptide that corresponds to the C-terminal amino acid sequence, a 15 kDa protein containing the extension was also detected in yeast.

【 授权许可】

Unknown