Direct photoaffinity labeling of soluble GTP‐binding proteins in Dictyostelium discoideum

FEBS Letters

Schleicher, Michael¹  Isenberg, Gerhard¹  Witke, Walter² 

[1] Max-Planck-Institute for Psychiatry 8033 Martinsriedl/Munich, FRG;Max-Planck-Institute for Biochemistry, 8033 Martinsriedl/Munich, FRG

关键词: Actin-binding protein (Dictyostelium) Photoaffinity labeling Nucleotide-binding protein;   

DOI  :  10.1016/0014-5793(86)80529-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Major cytoplasmic GTP-binding proteins in Dictyostelium discoideum were identified by direct photoaffinity labeling with [α-³²P]GTP. Three actin-binding proteins and a protein with an apparent molecular mass of 24 kDa (p24) could be labeled with [α-³²P]GTP. p24 binds to DEAE-cellulose, behaves like a monomer during gel filtration and was purified to homogeneity by GTP-affinity chromatography. In comparison to other nucleotide triphosphates the binding of GTP to p24 is highly specific.

【 授权许可】

Unknown