A developmentally regulated gene product from Dictyostelium discoideum shows high homology to human α‐L‐fucosidase

FEBS Letters

Westphal, M.¹  Gerisch, G.¹  Noegel, A.¹  Müller-Taubenberger, A.¹ 

[1] Max-Planck-Institut für Biochemie, 8033 Martinsried bei München, FRG

关键词: Fucosidase;    Development;    (Dictyostelium);   

DOI  :  10.1016/0014-5793(89)80280-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA library of poly(A⁺)-RNA has been prepared from membrane-bound polysomes of Dictyostelium discoideum and screened for clones hybridizing to mRNA species that encode developmentally regulated proteins. The clone investigated in this paper recognizes a 1.8 kb transcript that accumulates strongly between the growth phase and aggregation stage. Stimulation of cells with pulses of cAMP enhances the accumulation. The amino acid sequence derived from a complete cDNA and from a genomic clone displays extensive sequence identity to human liver α-L-fucosidase. The D. discoideum DNA sequence encodes a 50.5 kDa polypeptide with a hydrophobic signal peptide at the N-terminus. Antibodies against a synthetic peptide corresponding to amino acids 262–275 of the deduced protein sequence recognize a developmentally regulated 50 kDa protein in D. discoideum that is recovered in the particulate fraction.

【 授权许可】

Unknown