(Ca2+ + Mg2+)‐dependent ATPase mRNA from smooth muscle sarcoplasmic reticulum differs from that in cardiac and fast skeletal muscles

FEBS Letters

Wisnewsky, Claudine¹  Schwartz, Ketty¹  de la Bastie, Diane¹  Lompré, Anne-Marie¹ 

[1] INSERM U127, Hôpital Lariboisière, 41 Bd de la Chapelle, 75010 Paris, France

关键词: Sarcoplasmic reticulum;    (Ca2+ + Mg2+)-ATPase;    RNA blot analysis;    S1 nuclease mapping;    (Smooth muscle);    Ca2+-ATPase;    (Ca2+ + Mg2+)-dependent ATPase;    SR;    sarcoplasmic reticulum;   

DOI  :  10.1016/0014-5793(88)80794-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated some characteristics of the sarcoplasmic reticulum (Ca²⁺ + Mg²⁺)-dependent ATPase (Ca²⁺-ATPase) mRNA from smooth muscle using specific cDNA probes isolated from a rat heart cDNA library. RNA blot analysis has shown that the Ca²⁺-ATPase mRNA expressed in smooth muscle is identical in size to the cardiac mRNA but differs from that of fast skeletal muscle. S₁ nuclease mapping has moreover shown that the cardiac and smooth muscle isoforms possess different 3′-end sequences. These results indicate that a distinct sarcoplasmic reticulum Ca²⁺-ATPase mRNA is present in smooth muscle.

【 授权许可】

Unknown