Antibodies to the calmodulin‐binding Ca2+‐transport ATPase from smooth muscle

FEBS Letters

Wuytack, Frank¹  Casteels, Rik¹  Verbist, Jan¹  Schutter, Greet De¹ 

[1] Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, 3000 Leuven, Belgium

关键词: Calmodulin;    CaMg ATPase;    Erythrocyte;    Immunochemistry;    Sarcoplasmic reticulum;    Smooth muscle;    CaMg-ATPase;    (Ca2+ + Mg2+)-ATPase;    SDS;    Sodium-dodecylsulphate;    IgG;    immunoglobulin G;   

DOI  :  10.1016/0014-5793(83)80901-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antibodies were raised against a calmodulin-binding CaMg-ATPase (Ca²⁺-transport ATPase) from smooth muscle. The binding of these antibodies to a number of related Ca²⁺-transport ATPases was studied. Antibodies to the calmodulin-binding ATPase from porcine antrum (stomach) smooth muscle do not only bind to this CaMg-ATPase, but also to the corresponding enzyme in porcine erythrocytes. However, they do not bind to the CaMg-ATPase from sarcoplasmic reticulum of porcine skeletal muscle. The binding of these antibodies to the CaMg-ATPase of smooth muscle, does not inhibit the enzyme activity.

【 授权许可】

Unknown