Phenolic antioxidants: potent inhibitors of the (Ca2+ + Mg2+‐ATPase of sarcoplasmic reticulum

FEBS Letters

Daly, John W.¹  Spande, Thomas F.¹  Kauffman, Frederick C.²  Albuquerque, E.X.²  Sokolove, Patricia M.² 

[1] Laboratory of Bioorganic Chemistry. NIADDK, National Institutes of Health, Bethesda, MD 20205, USA;Department of Pharmacology and Experimental Therapeutics, University of Maryland, School of Medicine. Baltimore, MD 21201 USA

关键词: (Ca2+ + Mg2+)-ATPase;    Sarcoplasmic reticulum;    Phenolic antioxidant;    Tight-binding inhibitor;   

DOI  :  10.1016/0014-5793(86)80726-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bis(2-hydroxy-3-tert-butyl-5-methylphenyl)methane (bis-phenol) is the most potent inhibitor of the (Ca²⁺ + Mg²⁺-ATPase of skeletal muscle sarcoplasmic reticulum yet identified. The compound behaves as a reversible, tight-binding inhibitor with apparant K _i = 0.3 μM. Butylated hydroxytoluene, butylated hydroxyanisole, and 4-nonylphenol are also effective inhibitors. These observations are of particular interest in light of the widespread use of such phenolic antioxidants and stabilizers in the food industry and in the manufacture of rubbers and plastics and the ease with which the compounds are extracted into organic solvents.

【 授权许可】

Unknown