Epidermal growth factor‐induced phosphoinositide hydrolysis Modulation by protein kinase C

FEBS Letters

Cervini, R.²  Mantegazza, P.²  Zippel, R.¹  Vicentini, L.M.² 

[1] Department of General Physiology and Biochemistry, University of Milano, Milan, Italy;Department of Pharmacology University of Milano, Milan, Italy

关键词: Epidermal growth factor;    Inositol phosphate;    Phorbol ester;    (A431 cell);    InsPs;    inositol phosphates;    PPI;    polyphosphoinositides;    PDBu;    phorbol 12;    13-dibutyrate;    InsP1;    InsP2;    InşP3;    insP4;    inositol mono-;    bis-;    tris-;    tetrakis-phosphates;    respectively;    with assignment of phosphate locants where appropriate (e.g. Ins(1;    4;    5)P3);   

DOI  :  10.1016/0014-5793(88)80029-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A short-term treatment with phorbol 12,13-dibutyrate (PDBu) was found to inhibit totally the epidermal growth factor (EGF)-stimulated phosphoinositide hydrolysis in A431 cells, whereas long-term pretreatment with PDBu, which is known to down regulate protein kinase C, induced a greater accumulation of the EGF-triggered inositol phosphate accumulation, particularly of Ins(1,3,4,5)P₄. The increased Ins(1,4,5)P₃/Ins(1,3,4,5)P₄ formation in the PDBu long-term pretreated cells was coincident with the increased Ca²⁺ influx stimulated by EGF in the same cells. Since long-term pretreatment with PDBu was found to enhance the EGF signals, an explanation for the synergism between EGF and phorbol esters in the induction of DNA synthesis is provided.

【 授权许可】

Unknown