Biological activities of chemically synthesized N‐acetylneuraminic acid‐(α2→6) ‐monosaccharide analogs of lipid A

FEBS Letters

Achiwa, Kazuo²  Masuzawa, Toshiyuki¹  Ikeda, Kiyoshi²  Shimizu, Chikako²  Shimizu, Tadayori¹  Yanagihara, Yasutake¹ 

[1] Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, 2-2-1 Oshika, Shizuoka 422, Japan;Department of Medicinal Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 2-2-1 Oshika, Shizuoka 422, Japan

关键词: Synthetic lipid A analog;    N-Acetylneuraminic acid;    Mitogenic activity;    Lethal toxicity;   

DOI  :  10.1016/0014-5793(88)80593-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mitogenicity and lethal toxicity of chemically synthesized lipid A analogs, in which 2,3-acyloxyacylglucosamine-4-phosphate linked to tetraacetyl-N-acetylneuraminic acid (compound A-207) or to N-acetylneuraminic acid (compound A-307), were examined. Although the mitogenic activity of the synthetic compounds was weaker than that of bacterial LPS, doses of 10–50 μg/ml of A-207 and 5–10 μg/ml of A-307 were capable of increasing incorporation of [³H]thymidine into cultured spleen cells of C57BL/6 mice. Lethal toxicity of A-207 was observed at 10 μg/mouse in C57BL/6 mice sensitized with D-galactosamine hydrochloride. However, the attachment of tetraacetyl-N-acetylneuraminic acid or N-acetylneuraminic acid does not appear to enhance the biological activity of acyloxyacylglucosamine-4-phosphate.

【 授权许可】

Unknown