Isoproterenol produces a rapid increase in sialidase activity in rat heart tissue and cardiomyocyte‐derived H9c2 cells in culture

FEBS Letters

Saito, Megumi¹  Shiota, Tomoko¹  Ito, Masaki¹  Sakiyama, Katsuhiro¹ 

[1] Department of Clinical Pharmacology and Therapeutics, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan

关键词: Sialidase;    Isoproterenol;    3-Isobutyl-1-methylxanthine;    Sialic acid;    N-Acetylneuraminic acid;    Cardiomyocyte;    H9c2 cell;    Neu5Ac;    N-acetylneuraminic acid;    DMB;    1;    2-diamino-4;    5-methylenedioxybenzene;    IBMX;    3-isobutyl-1-methylxanthine;    4MU-Neu5Ac;    4-methylumbelliferyl-Neu5Ac;    Neu5Prop;    N-propanoylneuraminic acid;   

DOI  :  10.1016/S0014-5793(03)00359-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of isoproterenol on sialidase activity in rat cardiomyocytes were examined. Administration of isoproterenol to rats (0.2 or 2 mg/kg body weight) produced an increase in sialidase activity in total membrane fraction of heart tissue within 120 min (121±13% of the control at 120 min after administration of 0.2 mg isoproterenol/kg, n=5, P<0.05). Sialidase activity in cardiomyocyte-derived H9c2 cells was also increased by treatment with isoproterenol (10 μM) for 60 min. The effect of isoproterenol on sialidase activity was amplified by the addition of 3-isobutyl-1-methylxanthine (IBMX). Sialidase activity in H9c2 cells was elevated by treatment with dibutyryl cAMP plus IBMX without isoproterenol. The content of N-acetylneuraminic acid in cells decreased by 22% after treatment with isoproterenol plus IBMX. These results suggest that sialidase activity in rat cardiomyocytes is regulated by β-adrenergic stimulators via a cAMP-dependent process. The increased activity of sialidase may account for the reduction of sialic acid content of cells.

【 授权许可】

Unknown