Synthesis of a photoaffinity‐spin‐labeled derivative of ATP and its first application to F1‐ATPase

FEBS Letters

Schäfer, Günter²  Vogel-Claude, Pia¹  Trommer, Wolfgang E.¹ 

[1] Fachbereich Chemie, Universität Kaiserslautern, Erwin-Schrödinger-Straße, D-6750 Kaiserslautern, FRG;Institut für Biochemie, Medizinische Hochschule Lübeck, Ratzeburger Allee 160, D-2400 Lübeck 1, FRG

关键词: F1-ATPase;    Spin-labeled ATP;    Photoaffinity-spin-labeled ATP;    N3-ATP;    the 8-azido derivative of ATP;    SL-ATP or SL-N3-ATP;    C3′-(2;    2;    5;    5-tetramethyl-3-pyrroline-1-oxyl-3-carboxylic acid ester) of ATP and N3-ATP;    respectively;    HPLC;    high-performance liquid chromatography;    TLC;    thin-layer chromatography;   

DOI  :  10.1016/0014-5793(88)80878-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The synthesis of an ATP derivative is described, in which a spin-label is attached to the 3′-position of the ribose moiety and an azido group to C8 of the adenine ring (SL-N₃-ATP). Irradiation of this compound at 350 nm generates a nitrene, which will react with any functional group in its vicinity. SL-N₃-ATP exhibits a strongly immobilized ESR spectrum in a complex with F₁-ATPase from beef heart mitochondria. It was covalently incorporated into this enzyme. SL-N₃-ATP may be employed in ESR investigations under conditions in which non-covalent interactions are too weak for motionally restricted species to be easily observed.

【 授权许可】

Unknown