AT(D)PMg‐induced dissociation of the α3β3 complex of the F1‐ATPase from a thermophilic Bacillus PS3 into α1β1 heterodimers is prevented by mutation β(Y341C)

FEBS Letters

Hisabori, Toru¹  Kaibara, Chitose²  Odaka, Masafumi³  Yoshida, Masasuke² 

[1] Department of Biology, Yokohama City University, Seto, Yokohama 236, Japan;Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuta 4259, Yokohama 227, Japan;The Tokyo Metropolitan Institute of Medical Science, Honkomagome, Tokyo 113, Japan

关键词: F1-ATPase;    α3β3 complex of F1;    α1β1 heterodimer of F1;    ATP-induced dissociation;    CD;    circular dichroism;    HPLC;    high performance liquid chromatography;    TF1;    and EF1;    F1-ATPases from a thermophilic Bacillus strain PS3;    and E. coli;    respectively;   

DOI  :  10.1016/0014-5793(93)80618-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

AT(D)PMg induces dissociation of the α₃β₃ complex of F₁-ATPase from a thermophilic Bacillus strain, PS3, into the α₁β₁ heterodimers [(1991) Biochim. Biophys. Acta 1056, 279-284] but the location of the AT(D)PMg binding site responsible is not known. From the analysis of AT(D)PMg binding properties of the isolated mutant β subunit, β(y341c) and the stability of the α₃β(y341c)₃ complex in the presence of AT(D)PMg, we conclude that binding of AT(D)PMg to the Tyr-341 site of the β subunit(s) in the α₃β₃ complex triggers the dissociation of the α₃β₃ complex into the α₁β₁ heterodimers.

【 授权许可】

Unknown