Mutations in RCA1 and AFG3 inhibit F1‐ATPase assembly in Saccharomyces cerevisiae

FEBS Letters

Paul, Marie-Françoise¹  Tzagoloff, Alexander¹ 

[1] Department of Biological Sciences, Columbia University, New York, NY 10027, USA

关键词: F1-ATPase;    ATPase assembly;    AFG3 (YTA10);    RCA1 (YTA12);    Saccharomyces cerevisiae;    AAA protein-family is defined as TPase ssociated with diverse cellular ctivities [1];   

DOI  :  10.1016/0014-5793(95)00979-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The RCA1 (YTA12) and AFG3 (YTA10) genes of Saccharomyces cerevisiae code for homologous mitochondrial proteins that belong to the recently described AAA protein-family [Kunau et al. (1993) Biochimie 75, 209–224]. Mutations in either gene have been shown to induce a respiratory defect. In the case of rca1 mutants this phenotype has been ascribed to defective assembly of cytochrome oxidase and ubiquinol-cytochrome c reductase. In the present study we show that the respiratory defect of afg3 mutants, like that of rca1 mutants, is also caused by an arrest in assembly of cytochrome oxidase and ubiquinol-cytochrome c reductase. In addition to the absence of the respiratory complexes, rca1 and afg3 mutants exhibit reduced mitochondrial ATPase activity. As a first step to an understanding of the biochemical basis for the ATPase defect we have examined the assembly of the F₁ and F₀ constituents of the ATPase complex. We present evidence that the ATPase lesion stems at least in part from the failure of rca1 and afg3 mutants to assemble F₁. Although the mutants also display lower steady-state concentrations of some F₀ subunits, this could be a secondary effect of defective F₁ assembly.

【 授权许可】

Unknown