The 5′‐CA DNA‐sequence preference of daunomycin

FEBS Letters

Phillips, Don R.¹  Skorobogaty, Andrew¹  Reiss, James A.²  White, Robin J.² 

[1] Departments of Chemistry, La Trobe University, Bundoora, Victoria 3083, Australia;Departments of Biochemistry, La Trobe University, Bundoora, Victoria 3083, Australia

关键词: Daunomycin;    DNA;    Footprinting;    Sequence selectivity;    Transcription inhibition;   

DOI  :  10.1016/0014-5793(88)80877-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The DNA-sequence specificity of daunomycin was investigated by DNase I footprinting and an E. coli RNA polymerase transcription-inhibition assay. The 5′-CA sequence was identified as being the highest affinity binding site, although other modest affinity (5′-GC, CG, CT, TC, AC) and poor affinity sites (5′-AA, AT, TA) were also observed. The preference of daunomycin for 5′-CA nucleotide sequence suggests that its biological activity may arise from association with the 5′-CA-containing sequences thought to be associated with genetic regulatory elements in eukaryotes.

【 授权许可】

Unknown