Sequence selective binding of ditrisarubicin B to DNA: comparison with daunomycin

FEBS Letters

Fox, Keith R.²  Kunimoto, Setsuko¹ 

[1] Institute of Microbial Chemistry, 3-14-23 Kamiosaki, Shinagawa-ku, Tokyo 141, Japan;Dept of Physiology & Pharmacology, University of Southampton, Bassett Crescent East, Southampton S09 3TU, England

关键词: Daunomycin;    Ditrisarubicin;    Anthracycline;    Footprinting;    DNase I;   

DOI  :  10.1016/0014-5793(89)80747-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNase I footprinting has been used to examine the sequence selective binding of ditrisarubicin B, novel anthracycline antibiotic, to DNA. At 37°C no footprinting pattern is observed, the drug protects all sites from enzymic cleavage with equal efficiency. At 4°C a footprinting pattern is induced with low drug concentrations which is different from that produced by daunomycin. The best binding sites contain the dinucleotide step GpT (ApC) and are located in regions of alternating purines and pyrimidines.

【 授权许可】

Unknown