Absence of 7‐acetyl taxol binding to unassembled brain tubulin

FEBS Letters

Chenu, Jacques³  Guénard, Daniel¹  Wright, Michel²  Guéritte-Voegelein, Françoise¹  Takoudju, Martin² 

[1] Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, 91190 Gif-sur Yvette, France;Laboratoire de Pharmacologie et de Toxicologie Fondamentales, Centre National de la Recherche Scientifique, 205, route de Narbonne, 31077 Toulouse Cédex, France;SANOFI Recherche, 195, route d'Espagne, BP 1072, 31035 Toulouse France

关键词: Taxol;    Microtubule;    Tubulin;    Equilibrium dialysis;    Ac-taxol;    7-acetyl taxol;    Me2SO;    dimethyl sulfoxide;   

DOI  :  10.1016/0014-5793(88)80875-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of taxol on microtubule proteins at 0°C is controversial. In order to determine if taxol is unable to bind to unassembled tubulin, as has been hypothesized, the binding of [³H]acetyl taxol has been studied using equilibrium microdialysis. Ac-taxol bound to microtubules at 37°C and the binding remained stable when the temperature was lowered to 0°C. Ac-taxol bound also at 0°C to microtubules stabilized with rhazinilam. In contrast, there was no binding of Ac-taxol to unassembled tubulin, either free tubulin at 0°C or tubulin, complexed with several microtubule poisons, at 0 and 37°C.

【 授权许可】

Unknown