B‐ring of colchicine and its role in taxol‐induced tubulin polymerization

FEBS Letters

Biswas, Birendra B.²  Bhattacharyya, Bhabatarak²  Maity, Sankar²  Ghosh Choudhury, Goutam¹ 

[1] Department of Biology, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4Kl, Canada;Department of Biochemistry, Base Institute, 93/1 Acharya P.C. Rd, Calcutta 700 009, India

关键词: Taxol Tubulin Polymerization Colchicine binding;   

DOI  :  10.1016/0014-5793(86)80292-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Taxol-induced assembly of purified tubulin is not inhibited by the colchicine analogue 2-methoxy-5-(2',3',4'-trimethoxyphenyl)tropone. Colchicine analogues having intact A, C and B-rings (without NH-CO-CH₃) such as desacetamidocolchicine have also been found to be inactive. It has been observed that these two colchicine analogues are incorporated into polymers when incubated in the presence of taxol. Furthermore, preformed taxol-induced polymers of tubulin have been found to bind these two colchicine analogues. These results suggest that colchicine-binding domains on the tubulin molecule are mostly (if not completely) exposed in the taxol-induced polymers.

【 授权许可】

Unknown