Interaction of estramustine phosphate with microtubule‐associated proteins

FEBS Letters

Fridén, Bo²  Wallin, Margareta²  Deinum, Johanna¹ 

[1] Dept. of Medical Physics, University of Göteborg, Box 330 31, S-400 33 Göteborg, Sweden;Dept. Zoophysiology, University of Göteborg, Box 250 59, S-400 31 Göteborg Göteborg, Sweden

关键词: Microtubule;    Tubulin;    Taxol;    Estramustine phosphate;    Microtubule-associated protein;    Pipes;    piperazine-N;    N'-bis(2-ethanesulphonic acid);    MAPs;    microtubule-associated proteins;   

DOI  :  10.1016/0014-5793(85)80536-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have reported [(1984) Cancer Res., in press] that estramustine phosphate inhibits microtubule assembly and disassembled preformed microtubules. We now present evidence that estramustine phosphate inhibits microtubule assembly by binding to the microtubule-associated proteins. We have found that: (1) additional microtubule-associated proteins relieved the inhibition of assembly by estramustine phosphate; (2) ³H-labelled estramustine phosphate bound predominantly to the microtubule-associated proteins; and (3) the content of the microtubule-associated proteins was reduced in taxol reversed estramustine phosphate-inhibited microtubules.

【 授权许可】

Unknown