Concurrent measurements of the inhibition of ATP synthesis and alanine transport in intact cells of Rhodobacter capsulatus

FEBS Letters

Elferink, M.G.L.¹  Crielaard, W.¹  Myatt, J.F.²  Hellingwerf, K.J.¹  Jackson, J.B.²  Konings, W.N.¹ 

[1] Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands;Department of Biochemistry, University of Birmingham, PO Box 363, Birmingham B15 2TT, England

关键词: ATP synthesis;    Alanine transport;    Antimycin A;    Myxothiazol;    CCCP;    (Rb. capsulatus);    CCCP;    carboxylcyanide-m-chlorophenylhydrazone;    J ala;    initial rate of alanine uptake;    J p;    initial rate of ATP synthesis;   

DOI  :  10.1016/0014-5793(87)81121-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Myxothiazol, antimycin A and carboxylcyanide-m-chlorophenylhydrazone (CCCP) all had a more pronounced inhibitory effect on the rate of ATP synthesis than on the rate of alanine transport during illumination of intact cells of Rhodobacter capsulatus. CCCP had the most potent effect and antimycin A the least. The data suggest that the energy-input requirement for alanine transport is lower than that for ATP synthesis. A new test for direct interactions between the electron transport system and the ATP synthase and alanine transporter is applied to the data. Subject to reservations about the accuracy of rate measurement in intact bacterial cells and about the specificity of inhibition, the test reveals that the proton motive force is not the sole determinant of the rate of ATP synthesis and the rate of alanine transport.

【 授权许可】

Unknown