Mitochondrial formation of hydrogen peroxide is causally linked to the antimycin A‐mediated prevention of tert‐butylhydroperoxide‐induced U937 cell death

FEBS Letters

Brambilla, Liliana¹  Cantoni, Orazio¹ 

[1] Istituto di Farmacologia e Farmacognosia and Centro di Farmacologia Oncologica Sperimentale, Università di Urbino, Via S. Chiara 27, 61029 Urbino, Italy

关键词: Antimycin A;    2-heptyl-4-hydroxyquinoline N-oxide;    Complex III;    tert-butylhydroperoxide;    Cytotoxicity;    tB-OOH;    tert-butylhydroperoxide;    HQNO;    2-heptyl-4-hydroxyquinoline N-oxide;    SKF 525A;    α-phenyl-α-propylbenzeneacetic acid 2-[diethylamino] ethyl ester;    SSF;    strand scission factor;   

DOI  :  10.1016/S0014-5793(98)00764-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antimycin A and 2-heptyl-4-hydroxyquinoline N-oxide (HQNO), both of which bind to the same site of complex III, prevented U937 cell killing promoted by tert-butylhydroperoxide (tB-OOH). This cytoprotection was not directly caused by inhibition of electron transport or reduced formation of tB-OOH-derived toxic species, but rather appeared to be the consequence of a mechanism involving mitochondrial formation of hydrogen peroxide. Ubisemiquinone was most likely the electron donor allowing the formation of superoxides and, as a consequence, of hydrogen peroxide.

【 授权许可】

Unknown