The antimycin sensitivity of flash‐induced ATP synthesis in photosystem I‐enriched subchloroplast vesicles

FEBS Letters

Galmiche, J.M.¹  Kraayenhof, R.²  de Wolf, F.A.¹ 

[1] Département de Biologie, Service de Biophysique, CEN Saclay, 91191 Gif-sur-Yvette, France;Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands

关键词: Antimycin A;    ATP synthesis;    Cyclic electron transfer;    Single turnover activation;    Photosystem I;    Subchloroplast vesicle;    Chl;    chlorophyll;    DBMIB;    2;    5-dibromo-3-methyl-6-isopropyl-p-benzoquinone;    DCMU;    3-(3;    4-dichlorophenyl)-1;    1-dimethyl urea;    DNP-INT;    2-iodo-6-isopropyl-3-methyl-2′;    4;    4′-trinitrodiphenyl ether;    Δµ̃H+;    transmembrane difference of electrochemical potential;    FCCP;    carbonyl-cyanide-p-trifluoromethoxy-phenylhydrazone;    PS I or II;    photosystem I or II;   

DOI  :  10.1016/0014-5793(88)81279-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Photosystem I-enriched vesicles, derived from spinach thylakoids by mild digitonin treatment, reconstituted with native ferredoxin and redox-poised with NADPH and oxygen, display antimycin-sensitive cyclic photophosphorylation. The effects of 1–30 μM antimycin point to electron transfer inhibition: (i) they are cancelled after addition of 10 μM pyocyanin and (ii) they resemble the effects of 1–5 μM DBMIB, but differ from those of FCCP, nigericin or valinomycin. Some vesicle preparations fails to show antimycin sensitivity in the absence of pyocyanin [(1985) FEBS Lett. 192, 271–274] and have apparently lost the antimycin-sensitive component.

【 授权许可】

Unknown