Eukaryotic elongation factor Tu is present in mRNA‐protein complexes

FEBS Letters

Greenberg, Jay R.¹  Slobin, Lawrence I.² 

[1] Cell Biology Group, The Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545 USA;Department of Biochemistry, The University of Mississippi Medical Center, Jackson, MS 39216, USA

关键词: Elongation factor Tu;    Protein synthesis;    mRNA;    RNA-protein complex;    Cross-linking;    EF-1;    elongation factor 1;    EF-Tu;    prokaryotic elongation factor Tu;    eEF-Tu;    eukaryotic elongation factor Tu;    mRNP;    messenger ribonucleoproteins;    PAGE;    polyacrylamide gel electrophoresis;    1D;    one-dimensional;    2D;    two-dimensional;   

DOI  :  10.1016/0014-5793(87)80421-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By two-dimensional gel electrophoresis, partial peptide mapping, and antibody binding we have shown that eukaryotic elongation factor Tu is in close contact with mRNA in rabbit reticulocytes. It can be crosslinked to mRNA by irradiating both polysomes and 40–80 S mRNA-protein complexes with short-wave UV light. To our knowledge this is the first case in which a known translation factor has been shown to be associated with mRNA in native ribonucleoproteins.

【 授权许可】

Unknown