Cross‐reconstitution of isolated F1‐ATPase from potato tuber mitochondria with F1‐depleted beef heart and yeast submitochondrial particles

FEBS Letters

Glaser, E.¹  Norling, B.¹  Hamasur, B.¹ 

[1] Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, S-106 91 Stockholm, Sweden

关键词: Cross-reconstitution;    F1-ATPase;    H+-ATPase;    Oligomycin sensitivity;    (Plant mitochondrion;    Mammal;    Yeast);    Mops;    3-(N-morpholino)propanesulfonic acid;    Tris;    1;    3-bis(tris[hydroxymethyl]amino)propane;   

DOI  :  10.1016/0014-5793(87)80310-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cross-reconstitution of isolated potato mitochondrial F₁-ATPase with F₁-depleted beef heart and yeast submitochondrial particles is reported. Potato F₁ binds to the heterologous membrane and confers oligomycin sensitivity on the ATPase activity of the reconstituted system. Binding of F₁ is promoted by the presence of Mg²⁺ with the maximal stimulatory efrect at 20 mM. Mg²⁺ increase the sensitivity to oligomycin of the reconstituted system consisting of potato F₁ and yeast membranes, however, they do not influence oligomycin sensitivity of potato F₁ and beef heart membranes.

【 授权许可】

Unknown