A yeast gene coding for a putative protein kinase homologous to cdc25 suppressing protein kinase

FEBS Letters

Ohno, Shigeo¹  Aoshima, Miho¹  Matsumoto, Seiji²  Yahara, Ichiro²  Suzuki, Koichi¹ 

[1] >Departments of Molecular Biology and Cell Biology, The Tokyo Metropolitan Institute of Medical Science, Hon-Komagome, Bunkyo-ku, Tokyo 113, Japan;Cell Biology, The Tokyo Metropolitan Institute of Medical Science, Hon-Komagome, Bunkyo-ku, Tokyo 113, Japan

关键词: Protein kinase;    cyclic AMP-dependent protein kinase;    Protein kinase C;    cdc25 suppressor gene;    (Saccharomyces cerevisiae);   

DOI  :  10.1016/0014-5793(87)80386-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A yeast gene termed YKR coding for a putative protein kinase was isolated by using the cloned cDNA for rabbit protein kinase C as a hybridization probe. The encoded protein (YKR), composed of 380 amino acid residues, shows extensive sequence homology to serine/threonine-specific protein kinases from various species in the approx. 320 C-terminal amino acid residues, strongly suggesting that YKR is endowed with a protein kinase activity. The observed homologies to the cdc25 suppressing protein kinase from yeast, the catalytic subunit of mammalian cAMP-dependent protein kinase, and mammalian protein kinase C were 76, 48 and 37%, respectively. Gene replacement experiments showed that YKR itself is not essential for cell proliferation.

【 授权许可】

Unknown