Demonstration that LSTRA cells have an elevated level of proteins phosphorylated on tyrosine residues

FEBS Letters

Thom, Rebecca E.¹  Casnellie, John E.¹ 

[1] Department of Pharmacology and Cancer Center, The University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA

关键词: Tyrosine protein kinase;    Phosphotyrosine antibody;    (Lymphoid cell);    pp56lck;    tyrosine protein kinase of M r 56000 found elevated in LSTRA cells;    pp60src;    the cellular homologue of the transforming protein of the Rous sarcoma virus;    p210c-abl;    form of the Abelson tyrosine protein kinase expressed in K562 cells;   

DOI  :  10.1016/0014-5793(87)80200-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The LSTRA cell line has been shown to have an exceptionally high level of a tyrosine protein kinase (pp56^lck). We now report that LSTRA cells also have a much higher level of proteins phosphorylated on tyrosine residues in comparison to several other cell lines with normal levels of pp56^lck. The level of phosphotyrosine-containing proteins in LSTRA cells was comparable to that seen in K562 cells, a cell line known to have a constitutively active tyrosine protein kinase. These results provide evidence that LSTRA cells have an elevated level of in vivo tyrosine protein kinase activity, probably due to the overexpression and activation of pp56^lck.

【 授权许可】

Unknown