Phosphotyrosine‐containing proteins in Dictyostelium discoideum

FEBS Letters

Schweiger, Anton¹  Muhr, Angelika¹  Mihalache, Oana¹  Adrian, Iris¹ 

[1] Max-Planck-Institut für Biochemie, Am Klopferspitz, 8033 Martinsried bei München, FRG

关键词: Phosphotyrosine antibody;    Protein-tyrosine kinase;    Dictyostelium discoideum;   

DOI  :  10.1016/0014-5793(90)81007-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphotyrosine-containing proteins in Dictyostelium discoideum were detected by immunoblot analysis and inununoprecipitation using a monoclonal anti-phosphotyrosine antibody. The iodinated antibody recognized on bots a cluster of 205–220 kDa polypeptides and bands of 107 and 60 kDa. The 107 and 60 kDa polypeptides and, in addition, a 82 kDa one became phosphorylated on tyrosine when the immunoprecipitate was incubated with [γ-³²p]ATP. In preparations from differentiating cells the intensity of the label was increased in the 60 kDa band and decreased in the 107 and 205–220 kDa bands.

【 授权许可】

Unknown