Nicotinic acetylcholine receptors in cultured neurons from the hippocampus and brain stem of the rat characterized by single channel recording

FEBS Letters

Deshpande, S.S.³  Lunt, G.¹  Swanson, K.L.³  Wonnacott, S.¹  Aracava, Y.³  Rapoport, H.²  Albuquerque, E.X.³ 

[1] Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, MD 21201, USA;Department of Biochemistry, University of Bath, Bath BA2 7AY, England;Program of Molecular Pharmacology, Institute of Biophysics ‘Carlos Chagas Filho’, University Federal of Rio de Janeiro, Rio de Janeiro, Brasil

关键词: Anatoxin;    Acetylcholine;    Patch clamp;    Central nervous system;    Nicotinic acetylcholine receptor;    Ion channel kinetics;   

DOI  :  10.1016/0014-5793(87)80192-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Single channel recording techniques have been applied to neurons cultured from the hippocampus and the respiratory area of the brain stem of fetal rats in order to search for nicotinic acetylcholine receptors (nAChR) in the central nervous system. In addition to acetylcholine (ACh), the potent and specific agonist (+)-anatoxin-a was also used to characterize nicotinic channels. nAChRs were concentrated on the somal surface near the base of the apical dendrite, and in some patches their density was sufficient to record 2 or more channel openings simultaneously. Although a multiplicity of conductance states was also evident, the predominant population showed a single channel conductance of 20 pS at 10°C. Thus, these neuronal nAChRs resembled the embryonic or denervated-type nAChRs in muscle. However, channel opening and closing kinetics were faster than reported for similar conductance channels in muscle. Therefore the nicotinic channels described here are similar but not identical to those of the well-characterized muscle nAChR, in agreement with biochemical, pharmacological, and molecular genetic studies on brain AChR.

【 授权许可】

Unknown