Cromakalim, a vasodilator, differentially inhibits Ca2+ currents in NG108‐15 neuroblastoma × glioma hybrid cells

FEBS Letters

Ito, Yuji²  Miyamori, Isamu¹  Takeda, Ryoyu¹  Matsubara, Takao¹  Higashida, Haruhiro² 

[1] Departments of Internal Medicine, Kanazawa University School of Medicine, Takaramachi-13, Kanazawa 920, Japan;Departments of Biophysics, Kanazawa University School of Medicine, Takaramachi-13, Kanazawa 920, Japan

关键词: Cromakalim;    Patch clamp;    Ca2+ inward current;    T-type;    L-type;    (NG108-15 cell);   

DOI  :  10.1016/0014-5793(90)80217-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Extracellular perfusion with the antihypertensive agent cromakalim produced an inhibition of 22–66% in the low-threshold transient Ca²⁺ (T-like) current in NG108-15 hybrid cells. Cromakalim suppressed the high-threshold and long-lasting Ba²⁺ current (L-like Ca²⁺ current) by 29–73%, but had almost no effect on the high-threshold and inactivating Ba²⁺ current (N-like Ca²⁺ current). IC₅₀ for T-like and L-like currents was the same at about 100 μM. The inhibitory effect developed relatively fast and was reversible. These results indicate that cromakalim can selectively inhibit the activity of inward Ca²⁺ currents.

【 授权许可】

Unknown