Low‐conductance chloride channel activated by cAMP in the epithelial cell line T84

FEBS Letters

Elie, D.¹  Tabcharani, J.A.¹  Low, W.¹  Hanrahan, J.W.¹ 

[1] Department of Physiology, McGill University, 3655 Drummond St., Montréal, Québec H3G 1 Y6, Canada

关键词: Epithelial secretion;    Patch clamp;    Chloride channel;    Cystic fibrosis;    T84 cell line;    4;    4'-Diisothiocyanatostilbene-2;    2'-disulfonic acid inhibition;   

DOI  :  10.1016/0014-5793(90)81257-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the modulation and pharmacological properties of two anion channels in T₈₄ cells by recording single channel and transepithelial currents. One channel had an outwardly rectifying current-voltage I/V curve, was rarely active in cell-attached patches, and was unaffected by cAMP. The other channel had lower conductance (8.7 pS at 37°C) and a more ohmic I/V relationship. Exposure to cAMP increased the probability of observing low-conductance channel activity in cell-attached patches > 6-fold. Extracellular DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid) or IAA-94 (an indanyloxyacetic acid) inhibited the outward rectifier but did not affect the low-conductance channel or cAMP-stimulated transepithelial current. These results suggest the low-conductance Cl channel may contribute to apical membrane conductance during cAMP-stimulated secretion.

【 授权许可】

Unknown