Fibronectin‐binding 36 kDa protein in human fibroblasts

FEBS Letters

Salonen, Eeva-Marjatta¹  Vaheri, Antti¹ 

[1] Department of Virology, University of Helsinki, 00290 Helsinki, Finland

关键词: Fibroblast;    Fibronectin-binding protein;    Protein isolation;    36 kDa protein;    DOC;    deoxycholate;    EIA;    enzyme immunoassay;    PBS;    0.01 M phosphate-buffered;    0.15 M saline;    pH 7.4;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    Tween;    polyoxyethylene sorbitane monolaureate 20;   

DOI  :  10.1016/0014-5793(87)80960-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 36 kDa fibronectin-binding protein was identified from electrophoretically separated proteins of the deoxycholate-soluble fraction of cultured fibroblasts by blotting with fibronectin and using poly- or monoclonal antibodies and immunoperoxidase staining to detect the bound fibronectin. The 36 kDa protein was purified by preparative electrophoresis and used to raise specific antibodies. Solid-phase 36 kDa protein bound plasma and fibroblast fibronectins equally well. The 36 kDa protein is an amphipathic protein with pI 5.9. It is monomeric with a tendency to dimerize and appears to be distinct from the cell surface fibronectin receptors which interact with the Arg-Gly-Asp recognition site in the fibronectin molecule.

【 授权许可】

Unknown