Ff-nano, Short Functionalized Nanorods Derived from Ff (f1, fd or M13) Filamentous Bacteriophage

Frontiers in Microbiology

Sadia eSattar¹  Wesley X Wen²  Len F Blackwell²  Jasna eRakonjac²  Jenness M Guthrie³  Nicholas J Bennett⁴  James Frederick Conway⁵ 

[1] COMSATS Institute of Information Technology;Massey University;Science Haven;University of Alberta;University of Pittsburgh;

关键词: phage display;    fibronectin;    nanorod;    M13 phage;    Fibronectin-binding protein;    lateral flow;   

DOI  :  10.3389/fmicb.2015.00316

来源: DOAJ

【 摘 要 】

F-specific filamentous phage of Escherichia coli (Ff: f1, M13 or fd) are long thin filaments (860 nm x 6 nm).They have been a major workhorse in display technologies and bionanotechnology; however, some applications are limited by the high length-to-diameter ratio of Ff.Furthermore, use of functionalized Ff outside of laboratory containment is in part hampered by the fact that they are genetically modified viruses.We have now developed a system for production and purification of very short functionalized Ff-phage-derived nanorods, named Ff-nano, that are only 50 nm in length.In contrast to standard Ff-derived vectors that replicate in E. coli and contain antibiotic-resistance genes, Ff-nano are protein DNA complexes that cannot replicate on their own and do not contain any coding sequences.These nanorods show an increased resistance to heating at 70 °C in 1 % SDS in comparison to the full-length Ff phage of the same coat composition.We demonstrate that functionalized Ff-nano particles are suitable for application as detection particles in sensitive and quantitative dipstick lateral flow diagnostic assay for human plasma fibronectin.

【 授权许可】

Unknown