Accelerated Detection of Viral Particles by Combining AC Electric Field Effects and Micro-Raman Spectroscopy

Sensors

Matthew Robert Tomkins¹  David Shiqi Liao² 

[1] Department of Chemical Engineering Queen's University, Kingston, ON K7L 3N6, Canada;

关键词: micro-Raman spectroscopy;    AC electrokinetics;    dielectrophoresis;    microelectrodes;    optical biosensors;    M13 phage;   

DOI  :  10.3390/s150101047

来源: mdpi

【 摘 要 】

A detection method that combines electric field-assisted virus capture on antibody-decorated surfaces with the “fingerprinting” capabilities of micro-Raman spectroscopy is demonstrated for the case of M13 virus in water. The proof-of-principle surface mapping of model bioparticles (protein coated polystyrene spheres) captured by an AC electric field between planar microelectrodes is presented with a methodology for analyzing the resulting spectra by comparing relative peak intensities. The same principle is applied to dielectrophoretically captured M13 phage particles whose presence is indirectly confirmed with micro-Raman spectroscopy using NeutrAvidin-Cy3 as a labeling molecule. It is concluded that the combination of electrokinetically driven virus sampling and micro-Raman based signal transduction provides a promising approach for time-efficient and in situ detection of viruses.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.