期刊论文详细信息
FEBS Letters
Isolation of a potassium‐selective ion channel from the plasma membrane of the broad bean Vicia faba L
Zeilinger, C.1 
[1] Institut für Biophysik, Universität Hannover, Herrenhäuserstr. 2, 30419 Hannover, Germany
关键词: Plasma membrane;    86Rb+ transport (K+ channel);    Protein isolation;    Bilayer technique;    Vicia faba;    CHAPS;    3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate;    PMSF;    phenylmethylsulfonylfluoride;    Mega-9;    nonanoyl-N-methylglucamide;    TEA;    tetraethylammoniumchloride;    PEG;    polyethylene glycol;   
DOI  :  10.1016/0014-5793(94)00629-6
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Potassium ions are essential for plant nutrition and play an important role in osmoregulation and plant movement. Recent patch clamp investigations and molecular analysis have identified voltage-dependent potassium channels responsible for translocation of this ion across plant membranes. In order to characterize these transporters on the biochemical level, a potassium channel protein was isolated from Vicia faba under preservation of its functional integrity. Potassium channel activity was monitored by tracer flux and black lipid bilayer measurements. The capability for K+ transport copurified with a 67 kDa protein from V. faba plasma membranes. A monoclonal antibody directed against the 67 kDa protein blocked channel activity. Single channels with a unit conductance of 40 pS were highly selective for K+ over Na+.

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