FEBS Letters | |
Isolation of a potassium‐selective ion channel from the plasma membrane of the broad bean Vicia faba L | |
Zeilinger, C.1  | |
[1] Institut für Biophysik, Universität Hannover, Herrenhäuserstr. 2, 30419 Hannover, Germany | |
关键词: Plasma membrane; 86Rb+ transport (K+ channel); Protein isolation; Bilayer technique; Vicia faba; CHAPS; 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate; PMSF; phenylmethylsulfonylfluoride; Mega-9; nonanoyl-N-methylglucamide; TEA; tetraethylammoniumchloride; PEG; polyethylene glycol; | |
DOI : 10.1016/0014-5793(94)00629-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Potassium ions are essential for plant nutrition and play an important role in osmoregulation and plant movement. Recent patch clamp investigations and molecular analysis have identified voltage-dependent potassium channels responsible for translocation of this ion across plant membranes. In order to characterize these transporters on the biochemical level, a potassium channel protein was isolated from Vicia faba under preservation of its functional integrity. Potassium channel activity was monitored by tracer flux and black lipid bilayer measurements. The capability for K+ transport copurified with a 67 kDa protein from V. faba plasma membranes. A monoclonal antibody directed against the 67 kDa protein blocked channel activity. Single channels with a unit conductance of 40 pS were highly selective for K+ over Na+.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020299775ZK.pdf | 585KB | download |