Primary structure of the acidic carbohydrate chain of hemocyanin from Panulirus interruptus

FEBS Letters

Vliegenthart, J.F.G.¹  Kamerling, J.P.¹  Breg, J.¹  Kolsteeg, C.E.M.¹  van Kuik, J.A.¹ 

[1] Department of Bio-Organic Chemistry, Utrecht University, Transitorium III, PO Box 80.075, NL-3508 TB Utrecht, The Netherlands

关键词: Hemocyanin;    N-linked carbohydrate;    Sulfate;    (Panulirus interruptus);    Man;    D-mannopyranose;    GlcNAc;    2-acetamido-2-deoxy-D-glucopyranose;    GlcNAc-ol;    2-acetamido-2-deoxy-D-glucitol;   

DOI  :  10.1016/0014-5793(87)80370-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The N-glycosidic carbohydrate chains of hemocyanin from the spiny lobster Panulirus interruptus were liberated by hydrazinolysis of a pronase digest and subsequently reduced. Separation of the mixture of oligosaccharide-alditols by high-voltage paper electrophoresis resulted in a neutral (90%) and an acidic (10%) fraction. 500-MHz ¹H-NMR spectroscopy of the acidic fraction revealed a single component with the following novel structure:

【 授权许可】

Unknown